the PAD fabricated by the wax dipping method can be used for
quantitative assays in the human samples.
4. Conclusions
The wax dipping method is a simple, rapid, and inexpensive
method for fabrication of PADs. Other advantages of this pioneering
method are that there is no requirement for complicated
and expensive instruments or organic solvents. Therefore, this
technique provides an alternative and inexpensive platform for
fabrication of clinical diagnostic devices in developing countries.
A single dipping step can create microfluidic channels on paper
within 1 min. Good resolution of the hydrophilic channel of the
PAD was obtained (%CV
∼2–7%). Additionally, the width of the
hydrophilic channel was highly correlated with the width of the