In order to investigate the potential to induce gene mutations in bacteria, an Ames test was performed
according to OECD Test Guideline 471 (OECD, 1997a) and following Good Laboratory Practice
(GLP) in four strains of Salmonella typhimurium (TA1535, TA100, TA1537, TA98) and Escherichia
coli WP2uvrA, in the presence or absence of metabolic activation by S9 mix applying the “plate
incorporation assay”.