Four trials were carried out with the shrimp
P. vannamei: 12 aquaria (300 l of water in each) with
three replicates for each treatment and the Control were
used. The ingredients and chemical composition of the
basal diets used in the experiment were as described by
Deshimaru and Kuroki (1974) and Wang et al. (2002);
the basal diet formulation is given in Table 1. The
ingredients were mixed, extruded and air-dried at room
temperature, and kept at −20 °C.