After the TEM observation and the PEF sterilization mechanism supposition in the existed literature, at first we suppose the cell is spherical, and the cell membrane is considered to be filled with the insulation medium as an electric capacity. The normal cell membrane potential difference is probably 10 mV between membranes[10,11]. The electric charges distributing in the cell membranes resulting in the cell membrane potential difference, together with the field intensity and the cell radius, they become proportion. The increase of voltage between the cell membrane has caused cell membrane thickness reducing. If the field intensity is further increased, and voltage between the cell membrane reaches the critical voltage (approximately 1 V), it will brings on the electric breakdown. If we suppose that penetration can cause the cell membrane to have the pore, then we pour the electric conduction solution into the pore, all this will conduced the electric charge discharging, thus causes the cell membrane destruction. If eyelets produced in the cell membrane are very few, the electric breakdown will be reversible. Except higher than the critical field intensity and the long time function, there is no way can enable the cell membrane to have compared wide range of penetration. If both the size and quantity of the eyelet in the cell membrane are very big, the electric breakdown becomes irreversible, and the cell membrane structure will be completely destroyed[12-14].