The extraction procedure used to quantify the content of extractable
polyphenols and antioxidant activity was developed for
Larrauri, Rupérez, and Saura-Calixo (1997). It was used 5 g of pulp
in centrifuge tubes and extracted firstly with 20 ml of methanol/
water (50:50, v/v) leaving it in rest at room temperature for
60 min. The tubes were centrifuged at 15,000 rpm for 15 min and
the supernatant was recovered. Following that 20 ml of acetone/
water (70:30, v/v) was added to the residue at room temperature
for 60 min and centrifuged. The extract was created from the
mixture of the supernatant of methanol and acetone, in 50 ml
volumetric flask, filling with distilled water.
The extraction procedure used to quantify the content of extractablepolyphenols and antioxidant activity was developed forLarrauri, Rupérez, and Saura-Calixo (1997). It was used 5 g of pulpin centrifuge tubes and extracted firstly with 20 ml of methanol/water (50:50, v/v) leaving it in rest at room temperature for60 min. The tubes were centrifuged at 15,000 rpm for 15 min andthe supernatant was recovered. Following that 20 ml of acetone/water (70:30, v/v) was added to the residue at room temperaturefor 60 min and centrifuged. The extract was created from themixture of the supernatant of methanol and acetone, in 50 mlvolumetric flask, filling with distilled water.
การแปล กรุณารอสักครู่..
