Temperature affected PGE2 production in the cultured ampullary epithelial cells. PGE2 concentration was significantly higher at 40.5 °C than at 38.5 °C (Fig. 3A). On the other hand, elevated temperatures did not affect PGE2 production in the isthmic epithelial cells (Fig. 3B). The culture temperature had no effect on PGF2α production in either the ampullary cells or isthmic cells (Fig. 3C and D). In the experiments described below, the cells were incubated at 38.5 and 40.5 °C, because PGE2 production at these temperatures is significantly different.