2.4. Latency period and spawning performance
The duration between hormone injection and first
appearance of spawned eggs is termed as latency period.
After complete spawning, all eggs were collected and transferred
to separate incubation tanks for different treatment
groups for hatching. Total fecundity was determined by
counting the number of eggs in 1 mL and multiplied by the
total volume of released eggs. Relative fecundity was calculated
as total number of eggs/weight of female fish. Egg
size was also measured under the microscope.
Fertilization rate (%) was determined by calculating the
number of fertilized eggs/total number of eggs counted X
100 and hatching rate (%) was determined by the number
of eggs that hatched/total number of fertilized eggs X 100.