Procedure:
I. Preparation of Carbohydrate Fermentation Broth
Weigh and dissolve trypticase, Sodium chloride, and Phenol red in 100 ml distilled water and transfer into conical flasks.
Add 0.5% to 1% of desired carbohydrate into all flasks.
Insert inverted Durham tubes into all tubes, the Durham tubes should be fully filled with broth.
Sterilize at 1150 C for 15 minutes.
Important: Do not overheat the Phenol red Carbohydrate fermentation broth. The overheating will result in breaking down of the molecules and form compounds with a characteristic color and flavour. The process is known as caramelization of sugar (the browning of sugar).
Transfer the sugar into screw capped tubes or fermentation tubes and label properly.