The maximum amylase production (538 U/ml) was achieved when the temperature was around 32 °C at pH 7 by the strain SSII2. The production of amylase was found to enhance when hydrolyzed sago starch was added as a sole carbon source, yeast extract as a nitrogen source and cysteine as an amino acid. After optimizing, the isolates scale up studies were carried out in 5 L fermentor with sago starch waste as a sole carbon source. The production of the enzyme reached 2.72 mg/L after 12 h. After partial purification the concentration of alpha amylase was found to be 54.54 mg/L. The maximum amylase enzyme activity was obtained at the beginning of the stationary growth phase.