Enterobacteriaceae are widely distributed and found in soil,water,vegetationandtheintestinaltractofanimals. Examination of foods, ingredients and raw materials, for the presence of marker groups such as coliforms or total Enterobacteriaceae , is one of the most common tests in food microbiology laboratory, because of the relative speed and ease with which the tests can be accomplished. Enterobacteriaceae are gram-negative chemoautotrophs that possess both respiratory and fermentative metabolism. Glucose Agar medium is used in the presumptive identification of Enterobacteriaceae based on the fermentation observed in the medium (1). This medium is also recommended by ISO (2, 3) as a solid medium for the confirmation of Enterobacteriaceae .