a loopful of each sample was
inoculated into 30% FYP broth (FYP broth supplemented
with 300 g l21
D-fructose) and incubated aerobically on an
orbital shaker (120 r.p.m.) at 30 uC until visible growth
was observed. The 30% FYP broth was used for selection of
fructophilic LAB. Species of the genus Fructobacillus are
known to resist high osmotic pressure (Endo & Okada