Moreover, biosurfactant properties remained the same after
autoclaving at 121 ◦C for 15 min. The biosurfactant was also successful in a test to simulate microbial
enhanced oil recovery (MEOR). Mass spectrometry analysis showed that the surface active compound
was a surfactin, known as a powerful biosurfactant that is commonly produced by Bacillus species. The
production of a high-efficiency biosurfactant, under some physical and chemical conditions that resemble those experienced in an oil production reservoir, such as high salinities and temperatures, makes
TSBSO 3.8 an excellent candidate and creates good expectations for its application in MEOR.