Animal is cooled on ice to provide anesthesia (less stressful than
needle administration of anesthetic in very young animal).
2. Vasculature is rinsed using hand-held syringe and #30 needle with
warm room temperature saline, room temperature fixative (10 ml),
and then ice-cold fixative.
3. Brain is removed after perfusion and postfixed 4 to 6 h. Dissection
and cutting as above.