Introduction
Toluene had been the most popular organic solvent used in various industries in the past. It continues to be a leading solvent in many countries although its prevalence might have been somewhat reduced in recent years
(Ukai et al. 1997). The validity of urinary metabolites,especially that of hippuric acid has been questioned as improvements in occupational hygiene have successfully reduced toluene levels in workroom air, e.g., to less than 50 ppm, and more attention
is currently focused on urinary excretion of toluene itself rather than its metabolites For determination of toluene in urine, head-space gas chromatography (HS-GC) is convenient because the method does not require complex pretreatment such as cleaning of the sample on an appropriate column. Caution should be exercised, however, to prevent any loss of toluene from urine, e.g., adsorption of toluene on the septum of the HS bottle, or possible spoilage of urine during storage because no freezing is allowed.
Results of successful trials are described in this paper to show that a high-pressure liquid chromatography (HPLC) method is applicable to urinalysis for toluene in urine. Not only is the method hand-saving yet reliable, but it also allows storage of urine samples for at least1 week without any decrease in toluene concentration.The confounding effects of glycosuria observed in untreated
diabetics and prevention of the effects by the addition of methanol are also described.
IntroductionToluene had been the most popular organic solvent used in various industries in the past. It continues to be a leading solvent in many countries although its prevalence might have been somewhat reduced in recent years(Ukai et al. 1997). The validity of urinary metabolites,especially that of hippuric acid has been questioned as improvements in occupational hygiene have successfully reduced toluene levels in workroom air, e.g., to less than 50 ppm, and more attentionis currently focused on urinary excretion of toluene itself rather than its metabolites For determination of toluene in urine, head-space gas chromatography (HS-GC) is convenient because the method does not require complex pretreatment such as cleaning of the sample on an appropriate column. Caution should be exercised, however, to prevent any loss of toluene from urine, e.g., adsorption of toluene on the septum of the HS bottle, or possible spoilage of urine during storage because no freezing is allowed.Results of successful trials are described in this paper to show that a high-pressure liquid chromatography (HPLC) method is applicable to urinalysis for toluene in urine. Not only is the method hand-saving yet reliable, but it also allows storage of urine samples for at least1 week without any decrease in toluene concentration.The confounding effects of glycosuria observed in untreateddiabetics and prevention of the effects by the addition of methanol are also described.
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