Diabetic animals were divided into eight groups, each containing six animals. The bile samples at 100% concentration (crude bile content) and 50% diluted bile sample (Bile content:water for injection = 1:1) were administered intraperitoneally and orally in respective groups of mice in the dose of 200 mg/kg body weight. The blood glucose levels for each animal of each group were estimated after 4, 6, 24 and 48 hours of administration.