3.1. Comparison of pretreatment methods
The amount of lipid extracted by the Bligh and Dyer method from
N. gaditana after the different pretreatments considered for this study
(steam explosion, ultrasound, microwave and autoclaving) is shown
in Fig. 2. For comparison purpose, the extraction of lipid from the
fresh untreated microalgal sample was also performed. The values of
lipid extracted are given as a percentage of the dry ash-free (DAF)
weight of the starting microalgae.