DON production by F. graminearum 124 and F. culmorum 627
was preliminarily evaluated on both wheat and rice, in order to assess
the most critical condition in which to further investigate the effect of
Trichoderma spp. on mycotoxin production. Wheat or rice kernels
(50 g) were added to 22.5 ml distilled water and autoclaved at 121 uC
for 30 min in a 500 ml Erlenmeyer flask. Each flask was inoculated with three OA plugs (6 mm diameter) cut from the edge of actively
growing cultures. The incubation was carried out at 24±2 uC (12 h
light/12 h darkness) for 28 days.