In the present study, the authors isolated and developed the culture of endometrial epithelial cells from buffalo uterus as well as evaluated functional properties of epithelial cells. In primary culture, epithelial cells appeared cuboidal or columnar and showed contact inhibition at the stage of confluence. Protein and DNA concentrations were found to increase with the time in culture. PGF 2alpha concentrations declined from 7.25+-2.02 pg/micro g DNA on Day 3 of culture to 6.33+-1.80 pg/micro g DNA on Day 5 of culture and thereafter to 2.98+-1.09 pg/micro g DNA on Day 7 of culture in endometrial epithelial cells. It was concluded that buffalo endometrial epithelial cells could serve as an excellent model for studying the specific role of PGF 2alpha in maternal recognition of pregnancy and implantation.