Observation of pollen tubes in reciprocal interspecific crosses
For two lines of C. anguria, PI 147065 and PI 320052, and C.
metuliferus, PI 526242, pistils were pollinated with ‘MR-1’ pollen
under controlled temperature (28 ◦C). Pollens germinated and
pollen tubes grew straight in the style (Fig. 2a–c). Morphological
features of these pollen tubes were not different from those of
‘MR-1’ self-pollination (Fig. 2f). However, when ‘MR-1’ pistils were
pollinated with PI 147065, PI 320052 or PI 526242 at 28 ◦C, most
pollen tubes stopped elongation in the stigma after germination;
no further elongation was observed (Fig. 2d and e). Furthermore,
the callose was densely deposited in pollen tubes and pollen grains;
callose plugs were rare and unclear (Fig. 2d and e).
Observation of pollen tubes in reciprocal interspecific crossesFor two lines of C. anguria, PI 147065 and PI 320052, and C.metuliferus, PI 526242, pistils were pollinated with ‘MR-1’ pollenunder controlled temperature (28 ◦C). Pollens germinated andpollen tubes grew straight in the style (Fig. 2a–c). Morphologicalfeatures of these pollen tubes were not different from those of‘MR-1’ self-pollination (Fig. 2f). However, when ‘MR-1’ pistils werepollinated with PI 147065, PI 320052 or PI 526242 at 28 ◦C, mostpollen tubes stopped elongation in the stigma after germination;no further elongation was observed (Fig. 2d and e). Furthermore,the callose was densely deposited in pollen tubes and pollen grains;callose plugs were rare and unclear (Fig. 2d and e).
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