The samples were then eluted in graded ethanol series (30%, 50%, 70%, 90%, and 100%) for 5 min at each gradation and isoamyl acetate was used to remove the ethanol.
The samples were then eluted in graded ethanol series (30%, 50%, 70%, 90%, and 100%) for 5 min at each gradation and isoamyl acetate was used to remove the ethanol.