Fermentation of MG hydrolyzate
Yeast extract, malt extract, and peptone were added to the overlimed
grass hydrolyzate. No additional glucose was added. The
mixture was sterilized at 121 C for 15 min and allowed to cool
at room temperature. S. cerevisiae (2–10% v/v) from the previous
step was added to the hydrolyzate, and the solution was incubated
in a shaker at 30 C for 96 h. Samples from the solution were taken
out every 24 h for glucose and ethanol detection.