set as zero, which was obtained from industrial company resulting in zero cost for feedstock transportation. The feedstock was used directly without pretreatment to decrease operated cost. In addition, condense molasses composed microbial protein, amino acids, organic acids, vitamins and coenzymes, which were necessary for bacteria growth [24]. The equipment of biomass-derived gas production system included: biohydrogen bioreactor, biomethane reactor, environmental pollution prevention, desulphurization system, purification system, and biomass-derived gas storage. The prices of equipment were obtained from vendors in Taiwan