with an increase in volume ratio due to the reduction of its volume. The low enzyme recovery observed at high volume ratio (>2.28) was due to the precipitation (as the bottom phase reaching its solubility limit). Even though highest activity of 109.4 U/ml was observed at volume ratio of 3.6, it resulted in lower recovery 74.43% and purification (5.67) compared to those at volume ratio of 2.28. The highest activity recovery of
91.99% was observed with maximum purification of 6.69 fold was observed at volume ratio of 2.28.