The field experiment was conducted in Randomized
Block Design with nine treatment combinations replicated
three times. The size of the individual plots was
1 m 1.5 m and the plot–plot distance was 0.5 m. Recommended
doses of fertilizers (30:50:20 for N-P-K) were
applied. Muriate of potash was sprayed @ 3% solution at
the flower bud initiation stage between 30 and 35 days
after sowing with a hand sprayer. Field application of
T. harzianum isolate STA7 was done by broadcasting on the
surface of plots on the day before planting @ 2.0% (WAV) by
growing on autoclaved wheat grain. The procedure for the
preparation of inocula of Trichoderma was the same as
that described earlier for the preparation of inocula of
R. solani. Seedlings of ‘BARI-1’ strawberry variety were
collected from the Bangladesh Agricultural Research
Institute and three seedlings were transplanted in each
plot. Three replications were used for each treatment.
Provax-200 50WP was applied as foliar spray at 10 days
after transplanting using a hand sprayer. The Trichoderma
isolate STA7, mustard oil cake and Vitavax-200 was
applied in different combinations in nine different treatments
viz. T1 = pathogen + Trichoderma isolate STA7;
T2 = pathogen + Provax-200; T3 = pathogen + mustered oil
cake; T4 = pathogen + Trichoderma isolate STA7 + Provax-
200; T5 = pathogen + Trichoderma isolate STA7 + mustered
oil cake; T6 = pathogen + mustered oil cake + Provax-200;
T7 = pathogen + Trichoderma isolate STA7 + mustered oil
cake + Provax-200; T8 = healthy seedling in a sterilized soil
(healthy control); T9 = healthy seedlings in a pathogeninoculated
soil (diseased control). Data on the mortality of
seedlings were recorded at the vegetative and reproductive
stages. Diseased seedlings were counted every
alternate day and continued for 28 days after planting.
To determine the cause of death of the seedlings, the dead
seedlings were uprooted gently. The causal pathogens
associated with the dead seeds and seedlings were isolated
and identified for the confirmation of the causal agents.
The data were analyzed for ANOVA using MSTAT-C
program, and mean separations were performed by DMRT.