One mechanism of how this is achieved is the stronger polymerase-DNA bond, i.e. via the SSo-binding domain (Wang et al., 2004).
Certain compounds, such as BSA and Tween-20, have also been
shown to increase inhibitor tolerance when they are added to the
PCR buffer (Kreader, 1996). Since the buffer is proprietary, its composition is unknown to the users. Additionally, the inhibitors would
also have been diluted using the dilution protocol (Kitpipit et al.,
2014). As the direct PCR procedure requires no extensive and lengthy
pre-extraction and extraction step, this novel assay is therefore costeffective and rapid. It addresses the drawbacks of conventional PCRbased methods previously reported.