The proFgCatB3 cDNA was generated and subcloned into the pPICZaA vector using the primer pairs 50-GCTGAAGCTGAATTCAAGCCAAACTAC-30 and 50-ATCTTGGTAGACGCGGCCGCAGGTAATCCGGC-30 containing the EcoRI and NotI restriction endonuclease sites (underlined), respectively, using Platinum Pfx DNA polymerase (Invitrogen).