However, the use of only mtDNA as a genetic marker in molecular ecology and phylogeographic studies possibly leads to misinterpretation and may yield an incomplete evolutionary history of the target species. This may be due to mitochondrial recombination in population levels, mitochondrial introgression, and the indirect selection caused by symbionts. Thus, the combined used of mitochondrial and nuclear DNA, as opposed to the sole usage of mtDNA markers, could allow researchers to answer more complicated evolutionary questions about target organisms.