Thermal treatment per se induced an increase of the pH value, as
can be noticed in the difference of 0.31±0.05 units between cooked
and raw control muscles (T5). In a previous study, it was observed a
similar pH difference between cooked (70 °C) and raw Semitendinosus
beef muscles (Szerman et al., 2007). This increase would have been
caused by the thermal denaturation of meat proteins and consequent
exposure and ionization of the buried groups that took place during
cooking (Ledward, 1979).