The cylinder was shaken intermittently for 10 min and allowed to stand for 10–15 min. The ether layer was then suctioned of fusing a water suction device. The extraction using petroleum ether was repeated. Then, 5 mL of the extracted solution was pipetted into a 100-mL volumetric flask, and about 10 mL of the diluted solution was mixed with 0.5 mL of I2/KI solution(0.2 g I 2 and 2 g KI dissolved in 100 mL of water). The mixture was then adjusted to a final volume of 50 mL in a volumetric flask with diluted water. The iodine blank was prepared by adding 0.5 mL of iodine solution to 50 mL of distilled water.The solutions were read at 625 nm against the blank. Resultsare expressed as a percentage on dw. The insoluble amylose content was calculated by subtracting the apparent amylose content from the content of soluble amylose.