The extracts for the total phenolic content assay were obtained
by extracting 1 g of homogenized berries (Polytron PT 1200E Homogenizer)
with 10 ml of methanol at ambient temperature for 1 h
with constant shaking. The solution was filtered, and the residue
was repeatedly extracted with 10 ml of methanol for 1 h. Finally, the
extracts were combined and diluted to 100 ml with methanol.
Then, total phenolic compounds were determined using the
FolineCiocalteu reaction (Singleton, Orthofer, & Lamuela-Raventos,
1999), with Gallic acid as the standard. Results were expressed as
mg Gallic acid equivalents per 100 g FW.