Briefly, root sample
powder was suspended in 10% w/v TCA/acetone containing 1m MPMSF and 0.07w/v mercaptoethanol, and held at −20 ◦C for 1h. After centrifugation and rinse,
the vacuum dried pellets were dissolved in 800 l lysis
solution containing 7 Murea, 2M thiourea, 4% (w/v) 3-((3-
cholamidopropyl) dimethylammonium) -1-propanesulfonate
(CHAPS), 65mM dithiothreitol (DTT),1mM PMSF and 0.5% v/v
biolytes.