Liquefaction is an early step of starch hydrolysis which was functioned by α-amylase, an endo-enzyme which randomly hydrolyzes α-1,4 glycosidic linkages in starch molecule leading to the release of dextrins and oligosaccharides. This renders the product viscosity to obviously be reduced (10). High levels of dextrins and oligosaccharides are required since they will be further hydrolyzed by glucoamylase to yield glucose, a substrate for fermentation of lactic acid and other valuable products. Glucoamylase is a debranching enzyme which hydrolyzes α-1,4 and α-1,6 glycosidic bonds (10).However, it is known that enzymes will fully function only under their optimal conditions. Various factors are realized to affect -amylase function. Information obtained from the product data sheet of Novo Nordisk A/S, Denmark reviewed that the amounts of -amylase and CaCl2, pH and incubation temperature are among the most important factors. Calcium was said to improved α-amylasestability while pH and temperature are important physicalenvironments.