Rf value of each spot was measured 0.21 and 0.25, respectively.
Annona squamosa Linn of Family Annonaceae, commonly known as “Custard apple” is a bio-pesticidal agent of plant origin. About 2 kg fresh leaves of A. squamosa, collected from surrounding area of Vidisha was shade dried in laboratory and pulverized to powder, then used for extract isolation by using Soxhlet apparatus. Percentage yield of A. squamosa extract was calculated i.e. 12.90 % only. Preliminary phytochemical screening of the plant A. squamosa extract was done by applying various tests. It was observed that methanolic extracts was found to be strong positive for Saponins,Flavonoids, Phenolic compounds, Glycosides, Lipids, Proteins and Alkaloids and negative for steroids. Thin layer chromatography of Annona squamosa plant extract was done using Ethyl acetate: Chloroform: Methanol (8:1:1)