Protocol
Subjects rested for five minutes. After that, they exercised on a pendulum ergometer for twenty minutes.
They warmed up with thirty seconds of pedaling with no resistance. Then the machine was re-calibrated to
a setting of 1.3kp of resistance. Subjects were instructed to pedal at a frequency of 60rpm. After nineteen
and a half minutes more of exercise, the resistance was returned to zero for a one minute cool down followed
by five more minutes of complete rest. The measurements taken were heart rate, oxygen uptake, blood
pressure and oral temperature. Heart rate was measured at one-minute intervals throughout all trials
using an HR monitor(POLAR:S610i). A total of six breath samples per subject were collected using the
Douglas bag method when the subjects were at rest, and every five minutes during exercise and recovery
(ARCO SYSTEM:ARCO-2000). Each exhaled breath sample was collected into a Douglas bag for a period of
five minutes during the resting and recovery phases and for four two-minute periods during exercise, from
the third minute to the fifth, from the eighth to the tenth, from the thirteenth to the fifteenth, and from the
eighteenth to the twentieth minute. Blood pressure was measured at rest and at every five minutes during
exercise and recovery (YAMASU:ANEROID SPHYGMOMANOMETER). Oral temperature was measured
before exercising using the electronic clinical thermometer (OMRON:MC-672L).