The strains were stained by lactophenol-cotton blue, carbol fuchsin and seen under
phase contrast microscope. The colonies were checked for their colour, outline and other
features.
Determination of enzyme synthesizing ability
To detect their ability to assimilate various polysaccharides, the strains were grown
Int. J. Chem. Sci.: 9(2), 2011 649
in basal medium composed of (gL-1): peptone 0.9; (NH4)2HPO4 0.4; KCl 0.1;
MgSO4.7H2O (pH 7) at 28°C for 18 hours, supplemented with starch, carboxy methyl
cellulose, xylan and pectin (0.5) respectively. For in situ detection of amylase and cellulase
& xylanase activities, plates were flooded with iodine solution (I2 + KI) and congo red
solution respectively. The catalase activity was checked by dropping few drops of H2O2 on
the culture. The quantitative assay of cellulase, amylase, xylanase and pectinase was done by
dinitrosalicylic acid method12. For checking the efficacy of the strains to grow and produce
alcohol, each strain was cultivated in basal medium supplemented with various sugars and
organic and inorganic nitrogen sources.
The strains were stained by lactophenol-cotton blue, carbol fuchsin and seen under
phase contrast microscope. The colonies were checked for their colour, outline and other
features.
Determination of enzyme synthesizing ability
To detect their ability to assimilate various polysaccharides, the strains were grown
Int. J. Chem. Sci.: 9(2), 2011 649
in basal medium composed of (gL-1): peptone 0.9; (NH4)2HPO4 0.4; KCl 0.1;
MgSO4.7H2O (pH 7) at 28°C for 18 hours, supplemented with starch, carboxy methyl
cellulose, xylan and pectin (0.5) respectively. For in situ detection of amylase and cellulase
& xylanase activities, plates were flooded with iodine solution (I2 + KI) and congo red
solution respectively. The catalase activity was checked by dropping few drops of H2O2 on
the culture. The quantitative assay of cellulase, amylase, xylanase and pectinase was done by
dinitrosalicylic acid method12. For checking the efficacy of the strains to grow and produce
alcohol, each strain was cultivated in basal medium supplemented with various sugars and
organic and inorganic nitrogen sources.
การแปล กรุณารอสักครู่..
