The derivatization and extraction process of the method
described in this paper was based on the RIKILT HPLC-UV method
developed to detect the tissue-bound furazolidone metabolite AOZ.
However, the present method was extended to cover the other
nitrofuran metabolites of concern in food of animal origin (AMOZ,
SEM and AHD) with a higher sensitivity. The limit of detection
(LOD) of the RIKILT method was 2 mg/kg (Rhijn Van & Mudler,
2002)