or samples are presented in Fig. 1. MDA peaks were identified by
means of the standards at the retention time of 3.106 min. The calibration
curves obtained in the MDA concentration range
0.1, 0.2, 0.4, 0.8, and 1.6 lM showed good linear regression
(y = 38.316x 0.2538; r2 = 0.9989). MDA compounds in the Control,
Nitrite, and Mix samples were clearly separated in the chromatogram.
The areas of the MDA peak from groups Control,
Nitrite, and Mix corresponded to 0.354, 0.274, and 0.290 mg/kg
meat product, respectively (Table 1). MDA content of Control group
was similar to that of the control samples analysed by the method
of the MDA–DNPH adduct. Although the MDA concentrations in
groups Nitrite and Mix were significantly lower than the MDA level
of the Control group, the similarity of the MDA concentrations
among the three groups appeared to be higher than the similarity
observed with the TBA assay and the MDA–DNPH method. The
lower MDA content in groups Nitrite and Mix may be explained
by the antioxidant activity of the nitrite in the Nitrite samples
and the nitrite and phosphate in the Mix samples (Sebranek, 2009).