Multiplex polymerase chain reaction assay We developed a multiplex PCR assay that utilises three different pairs of primers (triplex) designed previously for Dsp (Laurila et al., 2010), Pba (Frechon et al., 1998) and Pcc/Pwa (Kang et al., 2003) for the detection of major SRE pathogens in potato plant samples. We propose using presented multiplex PCR assay according to the scheme shown in Fig. 1