Leaves of Centella asiatica (Centella) were analysed for their triterpene
composition and bioactivity such as collagen enhancement, antioxidant, anticellulite and
UV protection capacity properties. Triterpenes of Centella were measured using HPLCPAD
on an Excil ODS 5 μm (C18) column for the simultaneous determination of asiatic
acid, madecassic acid, asiaticoside and madecassoside. Centella was found to contain
significant amounts of madecassoside (3.10 ± 4.58 mg/mL) and asiaticoside
(1.97 ± 2.65 mg/mL), but was low in asiatic and madecassic acid. The highest collagen
synthesis was found at 50 mg/mL of Centella extracts. The antioxidant activity of Centella
(84%) was compared to grape seed extract (83%) and Vitamin C (88%). Its lipolytic
activity was observed by the release of glycerol (115.9 μmol/L) at 0.02% concentration.
Centella extracts exhibited similar UV protection effect to OMC at 10% concentration. In
view of these results, the potential application of Centella in food and pharmaceutical
industries is now widely open
Leaves of Centella asiatica (Centella) were analysed for their triterpenecomposition and bioactivity such as collagen enhancement, antioxidant, anticellulite andUV protection capacity properties. Triterpenes of Centella were measured using HPLCPADon an Excil ODS 5 μm (C18) column for the simultaneous determination of asiaticacid, madecassic acid, asiaticoside and madecassoside. Centella was found to containsignificant amounts of madecassoside (3.10 ± 4.58 mg/mL) and asiaticoside(1.97 ± 2.65 mg/mL), but was low in asiatic and madecassic acid. The highest collagensynthesis was found at 50 mg/mL of Centella extracts. The antioxidant activity of Centella(84%) was compared to grape seed extract (83%) and Vitamin C (88%). Its lipolyticactivity was observed by the release of glycerol (115.9 μmol/L) at 0.02% concentration.Centella extracts exhibited similar UV protection effect to OMC at 10% concentration. Inview of these results, the potential application of Centella in food and pharmaceuticalindustries is now widely open
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