In another set of experiments, RMO of T. alexandrinum
were assayed on culture agar plates of the homologous plant
juice (diluted 1:40) as well as nutrient and soil extract agar.
The culturing method was further modified by trying to adjust
the oxygen diffusion at the surfaces of inoculated agar plates.
A thin layer of semi-solid agar of the corresponding medium,
with or without inoculum, was overlaid on the agar surface.
cfu were statistically the lowest on nutrient agar and the highest
on plant juice. The application of a thin layer of overlay
semi-solid agar on top of the agar surface, post surface inoculation,
did significantly increase micro-colonies, particularly on
the plant juice agar plates