2.1.2. Root and bud induction
Mayer (1956) and Stichel (1959) cultured C. persicum tuber
explants and studied the formation of buds and roots and investigated
the effects of some substances such as adenine, nicotinic
acid, guanine and NAA on organogenesis. In fact, they were the
first to report on the in vitro culture of cyclamen. The first important
step is to control contamination, particular when using tuber
explants, since contamination restricts the success of in vitro culture.
Okumoto and Takabayashi (1969) cultured aseptic explants
from cyclamen tubers and investigated the effects of factors such as
curing (drying explants for the formation of a brown surface without
signs of microorganism activity), partial embedding of explants
in the medium, and temperature, on the development of cultures
and on the formation of roots and buds. Curing explants reduced
infection and also increased the rates and number of organs formed.
Geier (1977) demonstrated that contamination could be effectively
controlled by pretreatment of tuber segments with an antibiotic,
achromycin. Permanent culture with achromycin decreased the
viability of explants so Geier used achromycin as a pretreatment
and could control 75 or 100% of contamination at 50 and 100 mg l−1,
respectively.
2.1.2. Root and bud inductionMayer (1956) and Stichel (1959) cultured C. persicum tuberexplants and studied the formation of buds and roots and investigatedthe effects of some substances such as adenine, nicotinicacid, guanine and NAA on organogenesis. In fact, they were thefirst to report on the in vitro culture of cyclamen. The first importantstep is to control contamination, particular when using tuberexplants, since contamination restricts the success of in vitro culture.Okumoto and Takabayashi (1969) cultured aseptic explantsfrom cyclamen tubers and investigated the effects of factors such ascuring (drying explants for the formation of a brown surface withoutsigns of microorganism activity), partial embedding of explantsin the medium, and temperature, on the development of culturesand on the formation of roots and buds. Curing explants reducedinfection and also increased the rates and number of organs formed.Geier (1977) demonstrated that contamination could be effectivelycontrolled by pretreatment of tuber segments with an antibiotic,achromycin. Permanent culture with achromycin decreased theviability of explants so Geier used achromycin as a pretreatmentand could control 75 or 100% of contamination at 50 and 100 mg l−1,respectively.
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