5-CQA was then purified in a silica gel column from the CGA mixtures. The monitoring of 5-CQA purification (97% of purity) was made by TLC (Rf = 0.6) and HPLC, and its structure was confirmed by 1H and 13C NMR spectroscopy ( Table 2). The purified 5-CQA was identified by comparison with spectroscopic data reported by Budryn et al. (2009), Hernandez et al. (2009), and Bhatt (2011).