In this study, the food waste consisted of around 406 mg starch/g food waste and 112 mg protein/g food waste, respectively. The theoretical glucose production of 451.1 mg glucose/g food waste could be calculated based on the molar basis of starch hydrolysis. When the food waste mass ratio was 5% (w/v), around 434 mg glucose/g food waste (21.7 g/L glucose) could be produced within 24 h. The starch conversion efficiency of food waste reached 96.2% by using enzymatic hydrolysis. Furthermore, around 224 mg/L of FAN was produced in the food waste hydrolysate which may be compared to 4.32 g yeast extract (Leung et al., 2012). Therefore, the results obtained from this study suggested that the proposed combined bioprocess could be potentially used to produce hydrogen from food waste in practical application.