In Brazil, the maximum allowed limit for aflatoxins in any product intended for animal feeding is 50 ng g−1 (sum of the four analogues) (Brasil, 1988). For mature poultry, 100 ng g−1 (sum of the four analogues) are the maximum allowed level in the United States of America (USA) while in European Union (EU) it is 20 ng g−1 (AFB1) (FAO, 2004). Therefore, in the present study 86.7% and 93.3% (ic-ELISA) and 90% and 96.7% (HPLC analysis) of the feed samples intended for broilers showed aflatoxin levels below the maximum allowed levels in the EU and Brazil. For laying hen feed samples these rates were 60.6% and 90.9% (ic-ELISA) and 60.6% and 93.9% (HPLC analysis), respectively. No sample of either feed type showed levels above those permitted in the USA legislation.