but fairly attached at lower trypsin treatment i.e.,0.125%.
On the other hand, trypsinised (0.125) cells from dissociated tissues of kidney and brain exhibited poor to no attachment at higher trypsin (0.25%) concentration. Effect of trypsin concentration was found on subsequent growth and formation of monolayer during primary culture of cells. Although cells derived from heart,liver,testis,overy and embryo showed good growth characteristics resulting in proliferation of cell and formation of monolayer (confluency); cell form kidney, brain and fin either failed to attach or grow and proliferate, thus ruling out the possibility of subculture (table 1)