HSCCC chromatogram of 1.0 g of the crude mulberry anthocyanins (CMA). Two-phase solvent system: methyl tert-butyl ether–n-butanol–acetonitrile–water–trifluoroacetic
acid (1:3:1:5:0.001, v/v); stationary phase: upper organic phase; elution mode in the coil system: head to tail; flow-rate: 3.0 mL/min; detection wavelength:
l ¼ 520 nm; retention of stationary phase: 62%; I: unknown mixture, II: C3R (1), III: C3RGa (2), IV: C3G (3), V: C3Ga (4), VI: C7G (5).