organism and this includes the free-living amoebae. Decreasing population of amoebae due to the presence of heavy
metal will leads to imbalance of the natural ecosystem as amoebae act as predator for bacteria.
The presence of heavy metal in the aquatic environments makes them a suitable toxicant to be evaluated for their
effects towards amoebae. To date, there are only a few reports on cytotoxic and genotoxic effects of heavy metal on
amoebae like cadmium, mercury, lead and zinc on these free-living amoebae [7]. However, there are no study
focusing the effect of Mn on Acanthamoeba. Therefore, it is important to evaluate the effect of Mn towards
Acanthamoeba as it would indicate the presence of exceed level of Mn in aquatic environment.