A submersible pump placed in the tank ensured water homogeneity and water oxygenation was maintained above 80% air saturation via vigorous air bubbling. As fish lost equilibrium, they were quickly removed from the tank, identified (pit tag reading) and placed in a recovery tank at their original acclimation temperature. The corresponding time and temperature (incipient upper lethal temperature, IULT) was also recorded. As for HCT, less than 1% mortality was recorded in the days that followed TCT.