rotozoa through growth curve determination, antiprotozoal and cytotoxicity assays.
Results:The extract revealed antibacterial activities, inhibiting the growth of Staphylococcus
aureus, Bacillus cereus, Pseudomonas aeruginosa, Micrococcus luteus, and Salmonella
typhimurium. Antifungal assay showed that it inhibited Candida albicans. The antiprotozoal
assay against Trichomonas vaginalisand Entamoeba histolyticashowed that V. globosa can
inhibit the parasites, wherein the action can be comparable to metronidazole. With the in situcell
death detection kit, Trichomonas vaginalisand Entamoeba histolyticaexposed to V. globosaleaf
extract was observed to fluoresce simultaneously in red and yellow signals signifying apoptoticlike changes. Preliminary phytochemical screening revealed the chemical composition of plant
extract containing alkaloids, saponins, 2-deoxysugars, and hydrolysable tannins. Conclusions:
Thus, this study provides scientific evidence on the traditional use of V. globosa leaf extract in
treating microbial diseas