2. Materials and methods
2.1. Dye solutions
Remazol Blue (CAS number: 128416-19-3) that is a dye commonly used in the cotton textile industry in Turkey. It was supplied from Aytemizler Textile Co., Turkey, in pure form. The dye stock solution was prepared by dissolving the powdered dyestuff in dis-tilled water to a final concentration of 2% w/v. Appropriate volumes of the stock solutions were added to the media.